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KMID : 0545120170270081441
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 8 p.1441 ~ p.1448
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A Rapid and Efficient Screening Method for Antibacterial Compound-Producing Bacteria
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Hettiarachchi Sachithra Amarin
Lee Su-Jin
Lee Young-Deuk
Kwon Young-Kyung
De Zoysa Mahanama
Moon Song
Jo Eun-Young
Kim Tae-Ho
Kang Do-Hyung
Heo Soo-Jin
Oh Chul-Hong
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Abstract
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Antibacterial compounds are widely used in the treatment of human and animal diseases. The overuse of antibiotics has led to a rapid rise in the prevalence of drug-resistant bacteria, making the development of new antibacterial compounds essential. This study focused on developing a fast and easy method for identifying marine bacteria that produce antibiotic compounds. Eight randomly selected marine target bacterial species (Agrococcus terreus, Bacillus algicola, Mesoflavibacter zeaxanthinifaciens, Pseudoalteromonas flavipulchra, P. peptidolytica, P. piscicida, P. rubra, and Zunongwangia atlantica) were tested for production of antibacterial compounds against four strains of test bacteria (B. cereus, B. subtilis, Halomonas smyrnensis, and Vibrio alginolyticus). Colony picking was used as the primary screening method. Clear zones were observed around colonies of P. flavipulchra, P. peptidolytica, P. piscicida, and P. rubra tested against B. cereus, B. subtilis, and H. smyrnensis. The efficiency of colony scraping and broth culture methods for antimicrobial compound extraction was also compared using a disk diffusion assay. P. peptidolytica, P. piscicida, and P. rubra showed antagonistic activity against H. smyrnensis, B. cereus, and B. subtilis, respectively, only in the colony scraping method. Our results show that colony picking and colony scraping are effective, quick, and easy methods of screening for antibacterial compound-producing bacteria.
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KEYWORD
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Colony picking, colony scraping, broth culture, screening, antibacterial compounds
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